Released on: October 4, 2007, 8:32 am

Press Release Author: AnaSpec Inc.

Industry: Biotech

Press Release Summary: AnaSpec has introduced two new catalog antibodies:
Anti-Keratin-18 and Anti-Keratin-19.

Press Release Body: October 2, 2007 - San Jose, CA

AnaSpec has introduced two new catalog antibodies: Anti-Keratin-18 and Anti-Keratin-19.

Intermediate filaments (IFs), together with actin microfilaments and tubulin
microtubules, comprise the three major cytoskeletal networks that are found in most
eukaryotic cells.1 Keratins, which are the largest IF protein subgroup, can be
divided into the acidic type I (K9-K20) and basic type II (K1-K8) keratins.2
Typically, epithelial cells express at least one of each type, and the proteins
exist in the cells as noncovalent obligate heteropolymers. Keratin networks are
highly dynamic and reorganized during cell differentiation, mitosis and apoptosis,
and also play important cytoprotective and structural support roles for the cell.3,4
During apoptosis, the epithelial keratins 18/19 undergo caspase-mediated
proteolysis, leading to the disassembly of the nuclear envelope and cytoplasmic IF
networks.5 K18 and K19 undergo caspase-cleavage at the conserved Asp237 in their rod
domains.5 In contrast to other type I keratins, K18 is unique in that it includes
another caspase-cleavage site at Asp396 of the carboxy-terminal domain.6

Anti-Keratin-18
Rabbit anti-human K18 (IN-2) polyclonal antibody was raised against a synthetic
peptide corresponding to an internal sequence of human keratin-18. This epitope
affinity purified rabbit polyclonal antibody reacts predominantly with human
keratin-18. Western blot using HT29 cell lysate from human colonic carcinoma shows a
49 kDa immunoreactive protein. Human, mouse, and monkey reactive. Predicted to
detect dog, bovine, and frog keratin -18.

Anti-Keratin-19
Rabbit anti-human K19 (IN) polyclonal antibody was raised against a synthetic
peptide corresponding to an internal sequence of human keratin-19. This epitope
affinity purified rabbit polyclonal antibody reacts predominantly with human
keratin-19. Western blot using HT29 cell lysate from human colonic carcinoma shows a
45 kDa immunoreactive protein. Human, mouse, chimpanzee, and monkey reactive.
Predicted to detect rat and bovine keratin-19.

Company Info
AnaSpec, Inc. is a leading provider of integrated proteomics solutions to
pharmaceutical, biotech, and academic research institutions throughout the world.
With a vision for innovation through synergy, AnaSpec focuses on three core
technologies: peptides, detection reagents (dyes, assay kits, & antibodies), and
combinatorial chemistry.

For more information, visit www.anaspec.com

References:
1. Ku, NO. et al. Am. J. Physiol. 277, G1108 (1999).
2. Schweizer, J. et al. J. Cell. Biol. 174, 169 (2006).
3. Omary, MB. et al. et al. Trends Biochem. Sci. 31, 383 (2006).
4. Omary, MB. et al. N. Engl. J. Med. 351, 2087 (2004)
5. Ku, NO. et al. J. Biol. Chem. 272, 33197 (1997).
6. Ku, NO. and MB. Omary, J. Biol. Chem. 276, 26792 (2001).




Web Site: http://www.anaspec.com

Contact Details: AnaSpec Inc.
ping@anaspec.com